A semi-automated instrument for cellular oxidative potential evaluation (SCOPE) of water-soluble extracts of ambient particulate matter

Abstract. Several automated instruments exist to measure the acellular oxidative potential (OP) of ambient particulate matter (PM). However, cellular OP PM is still measured manually, which severely limits comparison between two types assays. Cellular assays could provide a more comprehensive assessment PM-induced stress, as they incorporate biological processes involved in PM-catalyzed reactive oxygen species (ROS) generation. Considering this need, we developed semi-automated instrument, first its kind, for measuring based on macrophage ROS assay using rat alveolar macrophages. The instrument named SCOPE – evaluation uses dichlorofluorescein diacetate (DCFH-DA) probe detect samples extracted water. capable analyzing batch six (including one negative and positive control) 5 h equipped operate continuously 24 with minimal manual intervention after every analysis, i.e., h. has high analytical precision assessed from both controls (coefficient variation (CoV)<17 %). results obtained were good agreement measurements tert-butyl hydroperoxide (t-BOOH) control (slope =0.83 vs. manual, R2=0.99) =0.83, R2=0.71). We further demonstrated ability analyze large number laboratory dataset intrinsic several compounds, such metals, quinones, polycyclic aromatic hydrocarbons (PAHs) inorganic salts, commonly known be present PM. This potentially useful future studies apportion contribution key chemical overall